Click image to see more details
Product Info Summary
| SKU: | A02250-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Nogo receptor/NgR1/RTN4R Antibody Picoband™
View all Nogo Receptor/NgR Antibodies
SKU/Catalog Number
A02250-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Nogo receptor/NgR1/RTN4R Antibody Picoband™ catalog # A02250-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Nogo receptor/NgR1/RTN4R Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02250-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Nogo receptor/NgR1/RTN4R recombinant protein (Position: P56-R416).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02250-2 is reactive to RTN4R in Human, Mouse, Rat
Applications
A02250-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
50.708kDa
Background of Nogo Receptor/NgR
Reticulon 4 receptor (RTN4R) also known as Nogo-66 Receptor (NgR) or Nogo receptor 1 is a protein which in humans is encoded by the RTN4R gene. This gene encodes the receptor for reticulon 4, oligodendrocyte myelin glycoprotein and myelin-associated glycoprotein. This receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Nogo Receptor/NgR1/RTN4R using anti-Nogo Receptor/NgR1/RTN4R antibody (A02250-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nogo Receptor/NgR1/RTN4R antigen affinity purified polyclonal antibody (Catalog # A02250-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nogo Receptor/NgR1/RTN4R at approximately 70 kDa. The expected band size for Nogo Receptor/NgR1/RTN4R is at 63 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RTN4R (Source: Uniprot.org, NCBI)
Gene Name
RTN4R
Full Name
Reticulon-4 receptor
Weight
50.708kDa
Superfamily
Nogo receptor family
Alternative Names
NgR; NgR1; Nogo R; Nogo receptor; Nogo-66 receptor; NogoR; NOGORUNQ330/PRO526; reticulon 4 receptor; reticulon-4 receptor; RTN4R RTN4R NGR, NOGOR reticulon 4 receptor reticulon-4 receptor|Nogo-66 receptor|UNQ330/PRO526|nogo receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RTN4R, check out the RTN4R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RTN4R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nogo receptor/NgR1/RTN4R Antibody Picoband™ (A02250-2)
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No publications found for A02250-2
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